Research Reconstitution Tool
TB-500 Reconstitution Calculator
Calculate the reconstitution concentration of TB-500 (10 mg vial). Set your bacteriostatic-water volume and read the mg/ml and the mass at every U100 syringe mark. Research Use Only.
U100 insulin syringe (1 ml). The liquid shade reflects the concentration; each mark is labelled with the mass it holds at that concentration — not an amount to administer.
Research Use Only. This tool calculates the concentration of a reconstituted laboratory solution and the mass each syringe mark holds at that concentration. It does not provide, recommend, or imply any amount to administer. For in-vitro research use only — not for human or veterinary use.
How this calculator works
Set the volume of bacteriostatic water you plan to add to the TB-500 vial. The tool divides the vial’s milligram strength by that volume to give the concentration in mg/ml, the mass in one U100 syringe unit, and a reference table mapping every syringe mark to the mass it holds at that concentration. Volume is the input and mass is the result — the calculator never works backward from a target amount.
TB-500 is supplied as a 10 mg lyophilized vial.
Frequently asked questions
What exactly does this tool calculate?
It calculates a property of the reconstituted solution: its concentration (mg/ml), the mass in one U100 unit, and the mass each syringe mark contains at that concentration. It does not provide or imply any amount to administer.
Does more water make the solution stronger or weaker?
Weaker. Concentration is milligrams divided by millilitres, so a larger water volume spreads the same mass across more liquid — lower mg/ml at every mark.
What water is used to reconstitute TB-500?
Bacteriostatic water — sterile water containing 0.9% benzyl alcohol as a preservative — is the standard laboratory reconstitution solvent. Add it slowly down the inside of the vial wall and let the powder dissolve without shaking.
How is the reconstituted solution stored?
Keep the reconstituted solution refrigerated at 2–8°C and protected from light. TB-500 is a larger peptide and is sensitive to freeze-thaw aggregation — aliquot into single-use portions before freezing. The lyophilized vial is stored at −20°C until reconstituted.
