Research Reconstitution Tool
GHK-Cu Reconstitution Calculator
How much bacteriostatic water to reconstitute GHK-Cu (50 mg vial)?
For a 50 mg GHK-Cu vial, the volume of bacteriostatic water you add sets the concentration: 1 ml gives 50 mg/ml; 1.5 ml gives 33.33 mg/ml; 2 ml gives 25 mg/ml; 2.5 ml gives 20 mg/ml; 3 ml gives 16.67 mg/ml. At the 2 ml reference volume the solution is 25 mg/ml (250 mcg per U-100 syringe unit). Research Use Only — these are solution concentrations, not amounts to administer.
| Bacteriostatic water | Concentration | Mass per U-100 unit |
|---|---|---|
| 1 ml | 50 mg/ml | 500 mcg |
| 1.5 ml | 33.33 mg/ml | 333 mcg |
| 2 ml | 25 mg/ml | 250 mcg |
| 2.5 ml | 20 mg/ml | 200 mcg |
| 3 ml | 16.67 mg/ml | 167 mcg |
How much bacteriostatic water to reconstitute GHK-Cu (100 mg vial)?
For a 100 mg GHK-Cu vial, the volume of bacteriostatic water you add sets the concentration: 1 ml gives 100 mg/ml; 1.5 ml gives 66.67 mg/ml; 2 ml gives 50 mg/ml; 2.5 ml gives 40 mg/ml; 3 ml gives 33.33 mg/ml. At the 2 ml reference volume the solution is 50 mg/ml (500 mcg per U-100 syringe unit). Research Use Only — these are solution concentrations, not amounts to administer.
| Bacteriostatic water | Concentration | Mass per U-100 unit |
|---|---|---|
| 1 ml | 100 mg/ml | 1 mg |
| 1.5 ml | 66.67 mg/ml | 667 mcg |
| 2 ml | 50 mg/ml | 500 mcg |
| 2.5 ml | 40 mg/ml | 400 mcg |
| 3 ml | 33.33 mg/ml | 333 mcg |
Each “mass per U-100 unit” figure is the amount one unit on a U-100 insulin syringe (1 unit = 0.01 ml) holds at that concentration — a property of the prepared solution, not an amount to administer.
Loads the vial’s milligram strength only.
U100 insulin syringe (1 ml). The liquid shade reflects the concentration; each mark is labelled with the mass it holds at that concentration — not an amount to administer.
How to reconstitute GHK-Cu
GHK-Cu is a copper-binding tripeptide (glycyl-L-histidyl-L-lysine complexed with Cu²⁺) supplied as a deep-blue lyophilized powder in 50 mg and 100 mg vials. Select the vial strength, then set the bacteriostatic-water volume; the selector loads the milligram value only. Add the water slowly against the vial wall — the powder dissolves into a characteristic blue solution. The calculator returns the concentration in mg/ml and the mass at every U100 mark for the size you chose.
The two vial sizes produce different concentrations at the same volume: at 2 ml the 50 mg vial gives 25 mg/ml and the 100 mg vial gives 50 mg/ml. Across 1–3 ml the 50 mg vial spans 50 to about 16.7 mg/ml and the 100 mg vial spans 100 to about 33.3 mg/ml.
GHK-Cu reconstitution — frequently asked questions
How much bacteriostatic water for a 50 mg or 100 mg GHK-Cu vial?
Your choice of 1–3 ml sets the concentration. At 2 ml the 50 mg vial is 25 mg/ml and the 100 mg vial is 50 mg/ml; the calculator shows the exact mg/ml and per-mark mass for your volume.
Why are there two GHK-Cu vial sizes?
GHK-Cu is offered in 50 mg and 100 mg for different research scales. At the same water volume the 100 mg vial produces twice the concentration of the 50 mg vial.
What does the blue color of reconstituted GHK-Cu indicate?
The blue is the copper(II) coordinated to the tripeptide. A marked change from the characteristic deep blue can indicate copper dissociation, so keep the solution protected from light.
How is reconstituted GHK-Cu stored?
Refrigerate at 2–8°C, protected from light; hold the lyophilized vial at −20°C until reconstituted. Aliquot before freezing to limit freeze-thaw cycles.
See every peptide calculator we offer, one per compound.
Research Use Only. This tool calculates the concentration of a reconstituted laboratory solution and the mass each syringe mark holds at that concentration. It does not provide, recommend, or imply any amount to administer. For in-vitro research use only — not for human or veterinary use.
